In bioanalytical laboratories, the measurement of alcohol and its metabolites is performed using techniques such as gas chromatography (GC) and liquid chromatography (LC) coupled with mass spectrometry (MS). These techniques offer high sensitivity and specificity, enabling the accurate quantification of ethanol, acetaldehyde, and acetate in blood, urine, or breath samples. The development of portable devices for on-site testing, such as breathalyzers, further illustrates the application of bioanalytical principles in real-world scenarios.