Various techniques can be employed to detect denaturation:
1. Spectroscopy: Changes in UV-Vis or fluorescence spectra can indicate denaturation. 2. Circular Dichroism (CD): CD can provide information on changes in secondary structure. 3. Differential Scanning Calorimetry (DSC): DSC measures the heat capacity changes associated with denaturation. 4. Gel Electrophoresis: Denatured proteins often migrate differently compared to their native forms.
