Specificity is typically assessed by evaluating the method's ability to differentiate and quantify the analyte in the presence of potential interferences. This is done through:
1. Blank Matrix Analysis: Analyzing a blank sample matrix to ensure no signal is detected in the absence of the analyte. 2. Spiked Matrix Analysis: Adding known quantities of the analyte to the matrix and checking for accurate recovery. 3. Cross-Reactivity Studies: Testing the method against structurally similar compounds or metabolites to ensure no cross-reactivity occurs.
