Despite their potential, there are several challenges associated with the use of CNTs: - Toxicity: Raw CNTs can be toxic to biological systems. Functionalization is necessary to mitigate this issue. - Cost: The synthesis and functionalization of CNTs can be expensive, limiting their widespread use. - Aggregation: CNTs tend to aggregate, which can affect their performance in bioanalytical applications. - Regulatory Hurdles: The use of nanomaterials in medical applications is subject to strict regulatory requirements.