Despite rigorous validation and QC practices, several challenges can affect the reliability of bioanalytical methods, including:
Matrix Effects: Interference from other substances in the biological sample can affect analyte measurement. Degradation: The instability of analytes over time or due to environmental factors can lead to inaccurate results. Technological Advances: Rapid advancements in technology require continuous updates and validation of methods. Regulatory Requirements: Adhering to stringent regulatory standards can be demanding but is necessary for ensuring reliability.
