The analysis of nucleic acids presents several challenges:
1. Sensitivity and Specificity: Techniques must be sensitive enough to detect low levels of nucleic acids and specific enough to differentiate between similar sequences. 2. Contamination: Samples can be easily contaminated, leading to inaccurate results. 3. Degradation: RNA, in particular, is prone to degradation, which can complicate analysis. 4. Data Interpretation: The large volumes of data generated, especially in sequencing, require advanced bioinformatics tools for accurate interpretation.
