The process of single cell sequencing involves several key steps. First, individual cells are isolated using techniques such as microfluidics, FACS (Fluorescence-Activated Cell Sorting), or laser capture microdissection. Once isolated, the genetic material (DNA or RNA) is extracted from each cell. This material is then amplified and sequenced using high-throughput sequencing technologies. The resulting data is analyzed using specialized bioinformatics tools to reconstruct the genomic or transcriptomic landscape of each cell.
