Protein quantification can be achieved using several methods:
Bradford Assay: This colorimetric assay measures protein concentration based on the binding of Coomassie Brilliant Blue dye to proteins. BCA Assay: Another colorimetric method that uses bicinchoninic acid for protein quantification. UV-Vis Spectroscopy: This technique measures the absorbance of protein solutions, typically at 280 nm, to estimate concentration. Fluorescence-Based Assays: These assays use fluorescent dyes or tags to quantify proteins with high sensitivity.
