Protein analysis presents several challenges, such as:
Sample Complexity: Biological samples often contain a complex mixture of proteins, making isolation and analysis difficult. Protein Stability: Proteins are sensitive to environmental conditions and can denature easily, complicating analysis. Low Abundance Proteins: Detecting and quantifying low-abundance proteins require highly sensitive techniques. Post-Translational Modifications: PTMs add another layer of complexity as they can significantly alter protein function and require specialized techniques for detection.