What are the Common Techniques Used in Protein Analysis?
There are several techniques commonly used in protein analysis, each with specific applications and strengths:
Mass Spectrometry (MS): MS is a powerful technique for identifying and quantifying proteins in complex mixtures. It provides detailed information on protein structure and post-translational modifications. Western Blotting: This technique uses antibodies to detect specific proteins in a sample. It's widely used for protein identification and quantification. ELISA: ELISA is a sensitive method for measuring the concentration of proteins, particularly in clinical and research settings. Chromatography: Techniques such as HPLC and affinity chromatography are used to separate and purify proteins based on their physical and chemical properties. Protein Microarrays: These are used for high-throughput analysis of protein interactions and expression levels.
