1. Literature Review: Before starting, it’s essential to review existing methods and understand the challenges and limitations. 2. Selection of Analytical Technique: Choose the appropriate technique such as HPLC, LC-MS/MS, or ELISA based on the analyte and matrix. 3. Optimization: Adjust the parameters (e.g., pH, temperature, mobile phase composition) to achieve the best separation and detection. 4. Validation: Validate the method by testing its accuracy, precision, sensitivity, specificity, and robustness. 5. Documentation and Standard Operating Procedures (SOPs): Document the entire process and create SOPs to ensure consistency.
