An HPLC system consists of several key components:
Pump: Delivers the mobile phase at a constant flow rate through the system. Injector: Introduces the sample into the mobile phase stream. Column: Packed with the stationary phase, where the separation of sample components occurs. Detector: Identifies and quantifies the separated components as they elute from the column. Common detectors include UV-Vis, fluorescence, and mass spectrometry. Data System: Collects and analyzes the data generated by the detector.
