Several advanced detection methods are pivotal in bioanalytical sciences:
1. Mass Spectrometry (MS): A powerful analytical technique that measures the mass-to-charge ratio of ions. It is widely used for identifying complex mixtures of proteins, peptides, and metabolites.
2. High-Performance Liquid Chromatography (HPLC): A technique for separating, identifying, and quantifying compounds in a mixture. It is often coupled with MS for enhanced detection capabilities.
3. Nuclear Magnetic Resonance (NMR) Spectroscopy: Utilized to determine the structure of organic compounds by observing the interactions of nuclear spins when placed in a magnetic field.
4. Enzyme-Linked Immunosorbent Assay (ELISA): A plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones.
5. Next-Generation Sequencing (NGS): A high-throughput method used to sequence entire genomes or specific areas of interest with high accuracy and speed.
