Advantages: - High Sensitivity and Precision: Enables accurate quantification of low-abundance molecules. - Versatility: Applicable to a wide range of biological samples and analytes. - Automation: Modern HPLC systems are highly automated, increasing throughput and reproducibility. - Coupling with Detectors: Enhances analytical capabilities when coupled with mass spectrometry or other detectors.
Limitations: - Cost: HPLC systems and consumables can be expensive. - Complexity: Requires skilled operators for method development and troubleshooting. - Sample Preparation: Often necessitates extensive sample preparation to avoid column clogging and maintain system performance.
