amplification

What are the Common Methods of Amplification?

The most well-known method of amplification is the Polymerase Chain Reaction (PCR). PCR utilizes thermal cycling to denature DNA, anneal primers, and extend new DNA strands with the help of a DNA polymerase enzyme. Other methods include:
- Reverse Transcription PCR (RT-PCR): Converts RNA into complementary DNA (cDNA) before amplification.
- Quantitative PCR (qPCR): Measures the quantity of DNA by incorporating fluorescent dyes.
- Isothermal Amplification: Amplifies DNA at a constant temperature, including techniques like Loop-mediated Isothermal Amplification (LAMP).

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