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amplification
What are the Common Methods of Amplification?
The most well-known method of amplification is the
Polymerase Chain Reaction (PCR)
. PCR utilizes thermal cycling to denature DNA, anneal primers, and extend new DNA strands with the help of a DNA polymerase enzyme. Other methods include:
-
Reverse Transcription PCR (RT-PCR)
: Converts RNA into complementary DNA (cDNA) before amplification.
-
Quantitative PCR (qPCR)
: Measures the quantity of DNA by incorporating fluorescent dyes.
-
Isothermal Amplification
: Amplifies DNA at a constant temperature, including techniques like
Loop-mediated Isothermal Amplification (LAMP)
.
Frequently asked queries:
What is Amplification?
Why is Amplification Important?
What are the Common Methods of Amplification?
How Does PCR Work?
What are the Applications of Amplification in Bioanalytical Sciences?
What are the Advantages and Limitations of PCR?
What are the Future Directions in Amplification Technologies?
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