How are Complex Biological Matrices Prepared for Analysis?
To address the challenges posed by complex biological matrices, various sample preparation techniques are employed:
1. Solid Phase Extraction (SPE): A technique used to isolate and concentrate analytes from complex matrices. 2. Liquid-Liquid Extraction (LLE): Involves partitioning analytes between two immiscible liquids to separate them from interfering substances. 3. Protein Precipitation: A method to remove proteins from biological samples, thereby minimizing matrix effects. 4. Filtration and Centrifugation: Used to remove particulates and concentrate analytes.
