Spiking is typically performed by adding a known concentration of the analyte to a blank sample, which is a sample containing all the components except the analyte of interest. The spiked sample is then processed and analyzed in the same manner as the actual samples. Key steps involved in spiking include:
Preparation of Spiking Solution: A concentrated solution of the analyte is prepared, often in a solvent compatible with the sample matrix. Addition to Sample: A calculated volume of the spiking solution is added to the blank sample to achieve the desired concentration. Mixing: The sample is thoroughly mixed to ensure homogeneity.
