Several techniques are commonly used in bioanalytical sciences:
Chromatography: Techniques like High-Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) are used to separate and analyze compounds in a mixture. Mass Spectrometry (MS): Often coupled with chromatography, MS is used to determine the molecular weight and structure of compounds. Spectroscopy: Methods such as UV-Vis, IR, and NMR spectroscopy are used to analyze the interaction of light with matter to identify and quantify molecules. Electrophoresis: Techniques like gel electrophoresis and capillary electrophoresis are used to separate DNA, RNA, and proteins based on their size and charge. Immunoassays: Techniques like ELISA and RIA use antibodies to detect and quantify specific proteins and other molecules.
