biomolecular analysis

How Does Mass Spectrometry Work in Biomolecular Analysis?

Mass spectrometry (MS) involves ionizing biomolecules and measuring their mass-to-charge ratio to identify and quantify them. The process includes:
Ionization: Biomolecules are ionized using techniques like Electrospray Ionization (ESI) or Matrix-Assisted Laser Desorption/Ionization (MALDI).
Mass Analysis: Ions are separated based on their mass-to-charge ratio using mass analyzers such as Time-of-Flight (TOF) or Quadrupole analyzers.
Detection: The separated ions are detected, and their abundance is measured to generate a mass spectrum.

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